By Bernhard Sonnleitner
With contributions by means of a number of specialists
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Additional info for Advances in Biochemical Engineering - Biotechnology. - Bioanalysis and Biosensors for Bioprocess Monitoring
24. Schematic design of a flow cytometer. The exciting light is created by one or more laser sources which is focused by means of mirrors and lenses to a small measuring space (typically in the order of the particles to analyze) in the flow channel. Whenever a particle passes this space, extinction, forward or side scatter of light, or emission of fluorescent light occurs. These different light qualities are separated by lenses and mirrors and quantified by detectors (usually photomultipliers) mounted in appropriate positions.
When exposed to the antigen, an antibody–antigen complex will be formed on the surface and shift the resonance frequency of the crystal proportionally to the mass increment which is, in turn, proportional to the antigen concentration. A similar approach is used with surface acoustic wave detectors  or with the surface plasmon resonance technology (BIAcore, Pharmacia). Generally, biosensors are tricky to handle. g. an enzyme or a living microorganism [208, 209], they principally cannot be sterilized.
G. when performed using a filter separator operated in bypass or using a sample bus system. A bypass behaves as a plug flow type reactor fraction where flow-dependent spatial gradients develop and where no inactivation can take place because the bulk of the bypassed aliquots is returned to the reactor.
Advances in Biochemical Engineering - Biotechnology. - Bioanalysis and Biosensors for Bioprocess Monitoring by Bernhard Sonnleitner