By Phyllis R. Brown, Eli Grushka
This thirty fourth quantity examines topics comparable to high-performance capillary electrophoresis; gasoline chromatography, matrix isolation, and infrared spectrometry; and statistical theories of height overlap in chromatography.
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Extra info for Advances in Chromatography, Volume 34
Monoclonal and polyclonal antibodies especially are gaining great importance as a new generation of drugs. As the majority of these antibodies are produced by recombinant organisms in cell cultures, sophisticated quality and purity control becomes essential. At the same time, the large-scale fractionation of human serum was optimized in recent years to meet the growing demand of specific proteins, such as immunoglobulins, clotting Page 10 factors, and so on. The extremely low concentration of some of these blood proteins gave rise to the need for improved fractionation and process control techniques.
0, 20 mM NaCl, 30 kV; detection, 200 nm. ) Page 14 16 to 1000 mg/mL, while linearity between the peak height and the protein concentration was observed only in the range 16 to 250 mg/mL . This difference is caused by overloading effects, which have shown to increase the dispersion of the sample zone, thus influencing the maximum height recorded but not the overall peak area [31,62]. Concomitantly, separation performance, especially the theoretical plate number, decreases exponentially with increasing injection time, for the contribution of the sample plug to the total variance of the signal can be neglected only at small sample plug lengths [59,61].
Yeung Low-Temperature High-Performance Liquid Chromatography for Separation of Thermally Labile Species David E. Henderson and Daniel J. O'Connor Kinetic Analysis of Enzymatic Reactions Using High-Performance Liquid Chromatography Donald L. Sloan Heparin-Sepharose Affinity Chromatography Akhlaq A. Farooqui and Lloyd A. Horrocks Chromatopyrography John Chih-An Hu Inverse Gas Chromatography Seymour G. Gilbert Volume 24 Some Basic Statistical Methods for Chromatographic Data Karen Kafadar and Keith R.
Advances in Chromatography, Volume 34 by Phyllis R. Brown, Eli Grushka