By Sameh Magdeldin
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Additional resources for Affinity chromatography
Coli will be described for a better comprehensibility. Different methods of exploitation of affinity interactions for the protein immobilization will be referred in the last part of the chapter. The importance of achieved results for the biotechnological practice will be summarized. 30 Affinity Chromatography 2. Immobilization: What, why, how? At the beginning of the topic describing immobilization techniques and their advantages we should explain the meaning of the word "immobilization" in this text.
All three proteins bind extensively with the IgG subclass. In general, protein A is more suitable for cat, dog, rabbit and pig IgG whereas protein G is generally more preferable when purifying mouse or human IgG. A combination of protein A and G is also applicable for purifying a wide range of mammalian IgG samples. e IgG, IgM, IgA, and IgE), protein L is suitable for the purification of different classes of antibodies. The binding characteristics of antibody binding proteins (A, G and L) to a variety immunoglobulin species is summarized in Table 2.
4 Immobilization by chitin-binding domain Chitin represents another example of suitable affinity support, which is among other nontoxic, biodegradable and commonly occurring in the nature and thus available at relatively low cost. g. from chitinase A1 of Bacillus circulans WL-12, serves as appropriate affinity tag in these cases. D-Hydantoinase can be mentioned as an example of an enzyme successfully produced with a chitin-binding domain affinity tag and immobilized on chitin support. , 2007; Krajewska, 2004; Terpe, 2003).
Affinity chromatography by Sameh Magdeldin