By Shan S. Wong
Since the ebook of the 1st variation of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, significantly multifunctional cross-linkers, were built and synthesized. The of completion of the human genome venture has opened a brand new region for learning nucleic acid and protein interactions utilizing nucleic acid cross-linking reagents, and advances have additionally been made within the region of biosensors and microarray biochips for the detection and research of genes, proteins, and carbohydrates. additionally, advancements in actual strategies with remarkable sensitivity and determination have facilitated the research of cross-linked items.
Updated to mirror the advances of the twenty first century, this ebook offers:
- An evaluation of the chemical ideas underlying the methods of cross-linking and conjugation
- A thorough record of cross-linking reagents released within the literature because the first variation, overlaying monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers
- Reviews of using those reagents in learning protein tertiary buildings, geometric preparations of subunits inside complicated proteins and nucleic acids, near-neighbor research, protein-to-protein or ligand–receptor interactions, and conformational adjustments of biomolecules
- Discusses the appliance of immunoconjugation for immunoassays, immunotoxins for designated treatment, microarray expertise for research of varied biomolecules, and reliable country chemistry for immobilizations
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Additional info for Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition
82. , Principles of Nucleic Acid Structure, Springer-Verlag, New York, 1983. 83. Volkov, S. , Some aspects of the DNA hypochromic effect theory, Int. J. , 16, 119, 1979. 84. , Molecular mechanisms in nucleic acid photochemistry. I. Sensitized photochemical splitting of thymine dimer, J. Am. Chem. , 88, 6113, 1966. 85. Pashev, I. , Dimitrov, S. , Crosslinking proteins to nucleic acids by ultraviolet laser irradiation, Trends Biochem. , 16, 323, 1991. 86. Nikogosyan, D. , Two-quantum UV photochemistry of nucleic acids: Comparison with conventional low-intensity UV photochemistry and radiation chemistry, Int.
L. 21 Photochemistry of nucleic acid bases. 22 Photochemical dimerization of thymine in DNA. DNA 29 Review of Protein and Nucleic Acid Chemistry which transfers an electron to a neighboring thymine by abstracting a proton. The two radicals then combine to form a covalent bond. Similar photoactivated reactions can cross-link the nucleic acids to proteins as will be discussed later in this book. 23). These spontaneous reactions are of great importance in mutagenesis88 but of little or no application in cross-linking nucleic acids to proteins.
139. 3. Duggleby, K. G. , A competitive labeling method for the determination of the chemical properties of solitary functional groups in proteins, Biochemistry, 14, 5168, 1975. 4. Schewale, J. G. , Effects of Fe3+ binding on the microenvironments of individual amino groups in human serum transferrin as determined by different kinetic labeling, J. Biol. , 257, 9406, 1982. 5. , Nolte, R. , and Zannis, V. , 5, 3495, 1986. 6. Tskhovrebova, L. , Titan: Properties and family relationships, Nat. Rev.
Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition by Shan S. Wong