Shan S. Wong's Chemistry of Protein and Nucleic Acid Cross-Linking and PDF

By Shan S. Wong

ISBN-10: 084937491X

ISBN-13: 9780849374913

Since the ebook of the 1st variation of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, significantly multifunctional cross-linkers, were built and synthesized. The of completion of the human genome venture has opened a brand new region for learning nucleic acid and protein interactions utilizing nucleic acid cross-linking reagents, and advances have additionally been made within the region of biosensors and microarray biochips for the detection and research of genes, proteins, and carbohydrates. additionally, advancements in actual strategies with remarkable sensitivity and determination have facilitated the research of cross-linked items.

Updated to mirror the advances of the twenty first century, this ebook offers:

  • An evaluation of the chemical ideas underlying the methods of cross-linking and conjugation
  • A thorough record of cross-linking reagents released within the literature because the first variation, overlaying monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers
  • Reviews of using those reagents in learning protein tertiary buildings, geometric preparations of subunits inside complicated proteins and nucleic acids, near-neighbor research, protein-to-protein or ligand–receptor interactions, and conformational adjustments of biomolecules
  • Discusses the appliance of immunoconjugation for immunoassays, immunotoxins for designated treatment, microarray expertise for research of varied biomolecules, and reliable country chemistry for immobilizations

Show description

Read or Download Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition PDF

Best clinical chemistry books

Read e-book online Analytical Instrumentation: Performance Characteristics and PDF

There's an expanding desire for analysts to appreciate and manage to quantify the functionality of analytical tools, particularly with recognize to the following:* specifying apparatus for buy* estimating uncertainties in intrumental measurements* quantifying and demonstrating functionality qualityThis textual content hyperlinks jointly an figuring out of functionality features with an appreciation of the restrictions imposed via device layout, resulting in the interaction of the validation and qualification methods inside caliber coverage structures.

Download e-book for kindle: Bioactive Natural Products (Part J) by Atta-ur-Rahman

Typical items play an imperative and ongoing position in selling a variety of elements of medical development, and lots of points of easy examine courses are in detail with regards to typical items. the importance, for this reason, of the twenty ninth quantity within the experiences in normal Product Chemistry sequence, edited through Professor Atta-ur-Rahman, can't be over priced.

Organic Synthesis in Water by Paul A. Grieco (auth.) PDF

Using water as a medium for selling natural reactions has been quite ignored within the improvement of natural synthesis, although it's the solvent within which just about all biochemical approaches happen. Chemists have only in the near past began to relish the big power water has to provide within the improvement of latest artificial reactions and techniques, the place it may well provide merits in either precise chemistry and lowered environmental influence.

Additional info for Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition

Example text

82. , Principles of Nucleic Acid Structure, Springer-Verlag, New York, 1983. 83. Volkov, S. , Some aspects of the DNA hypochromic effect theory, Int. J. , 16, 119, 1979. 84. , Molecular mechanisms in nucleic acid photochemistry. I. Sensitized photochemical splitting of thymine dimer, J. Am. Chem. , 88, 6113, 1966. 85. Pashev, I. , Dimitrov, S. , Crosslinking proteins to nucleic acids by ultraviolet laser irradiation, Trends Biochem. , 16, 323, 1991. 86. Nikogosyan, D. , Two-quantum UV photochemistry of nucleic acids: Comparison with conventional low-intensity UV photochemistry and radiation chemistry, Int.

L. 21 Photochemistry of nucleic acid bases. 22 Photochemical dimerization of thymine in DNA. DNA 29 Review of Protein and Nucleic Acid Chemistry which transfers an electron to a neighboring thymine by abstracting a proton. The two radicals then combine to form a covalent bond. Similar photoactivated reactions can cross-link the nucleic acids to proteins as will be discussed later in this book. 23). These spontaneous reactions are of great importance in mutagenesis88 but of little or no application in cross-linking nucleic acids to proteins.

139. 3. Duggleby, K. G. , A competitive labeling method for the determination of the chemical properties of solitary functional groups in proteins, Biochemistry, 14, 5168, 1975. 4. Schewale, J. G. , Effects of Fe3+ binding on the microenvironments of individual amino groups in human serum transferrin as determined by different kinetic labeling, J. Biol. , 257, 9406, 1982. 5. , Nolte, R. , and Zannis, V. , 5, 3495, 1986. 6. Tskhovrebova, L. , Titan: Properties and family relationships, Nat. Rev.

Download PDF sample

Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition by Shan S. Wong


by Joseph
4.0

Rated 4.66 of 5 – based on 8 votes