By Dean F. Martin and Barbara B. Martin
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Extra resources for Column Chromatography
G. , Cocker D. R. & Purvis-Roberts K. L. Determination of methylamines and trimethylamine-N- oxide in particulate matter by non-suppressed ion chromatography. Journal of Chromatogra‐ phy A; 2010; 1217(13) 2070-2073.  Moldoveanu S. C. & Kiser M. Gas chromatography/mass spectrometry versus liquid chromatography/fluorescence detection in the analysis of phenols in mainstream cig‐ arette smoke Journal of Chromatography A 2007; 1141(1) 90-97. , Stokke T. , Jgensen, K. B. & Beyer J. Characterization of al‐ kylphenol metabolites in fish bile by enzymatic treatment and HPLC-fluorescence analysis Chemoshpere 2008; 71(7) 1392-1400.
4. Buffer In ion exchange chromatography, pH value is an important parameter for separation and can be controlled and adjusted carefully by means of buffer substances . In order to prevent variation in matrix and protein net charge, maintenance of a constant mobile phase pH during separation is essential to avoid pH changing which can occur when both protein and exchanger ions are released into the mobile phase . By means of buffer substances pH value can be controlled and adjusted. Concentration of H+ and the buffering component influence the protein binding to the stationary phase, chromatographic resolution and structural as well as functional integrity of the protein to be separated.
Zhao et al.  proposed a simple and ecofriendly ion chromatographic method for the determination of Hippuric acid (HA) in human urine (see Figure 12). Hippuric acid is a kind of metabolite of toluene in human body, therefore, HA is a physiological component of human urine if toluene was inhaled. The content of HA in human urine actually is confirmed as a diagnostic marker of exposure to toluene . 5772/55652 toluene lead to a series of diseases such as acute and chronic respiratory effects, functional alterations of the central nervous system, mucous and dermal irritations, and chromosome aberrations..
Column Chromatography by Dean F. Martin and Barbara B. Martin