By Jorge J. Yunis
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Additional info for Human Chromosome Methodology
1 9 5 6 ) . The chromosomes of man. Nature (London) 1 7 8 , 1 0 2 0 - 1 0 2 3 . London Conference on the Normal Human Karyotype ( 1 9 6 3 ) . Cytogenetics 2, 264-268. Mutton, D. E . , and Daker, M. G. ( 1 9 7 3 ) . Pericentric inversion of chromosome 9. Nature (London) New Biol. 2 4 1 , 80. Nowell, P. C , and Hungerford, D. A. ( 1960 ) . A minute chromosome in human chronic granulocytic leukemia. Science 1 3 2 , 1 4 9 7 . O'Riordan, M. L . , Robinson, J . , Buckton, Κ. E . , and Evans, H. J .
The slides are merely left in fixative ( 3 : 1 methanol : acetic acid) for 1 hour before exposure to atebrin. D. Microscopy Probably any microscope setup for fluorescence photography can be used. However, since in our laboratory we have had experience only with Zeiss fluorescence microscopes, the following applies to their products. The standard fluorescence microscope and a photomicroscope equipped with an HBO 200 W / 4 mercury burner and an ultra-dark 56 I R E N E A. UCHIDA AND C. C. L I N field condenser for transmitted illumination are in general use and are equally good.
Nature (London) New Biol. 2 3 8 , 1 2 2 - 1 2 4 . Borgaonkar, D. , and Hollander, D. H. ( 1 9 7 1 ) . Quinacrine fluorescence of the 2 3 0 , 52. human Y chromosome. , Zech, L . , and Johansson, C. ( 1 9 7 0 a ) . Analysis of the human metaphase chromosome set by aid of DNA-binding fluorescent agents. Exp. Cell Res. 6 2 , 4 9 0 - 4 9 2 . 6 . , Lindsten, J . , and Zech, L . ( 1 9 7 0 b ) . Identification of the Philadelphia chromosome as a number 2 2 by quinacrine mustard fluorescent analysis. Exp.
Human Chromosome Methodology by Jorge J. Yunis