By Denis Faure, Dominique Joly
Bringing jointly the newest methodological and medical growth within the a variety of examine components within the box of Environmental Genomics, this e-book discusses the characterization of the constitution and dynamics of existence, the examine of the evolution and edition of genes and genomes, the research of degraded and/or outdated DNA, and the practical and genomic ecology of populations and communities. It additionally considers entry to the construction and sharing of NGS information and the standard of this knowledge. because the made from the collective dialogue of the lively French clinical neighborhood, the booklet offers not just the most recent applied sciences within the improvement of latest sequencing tools, but additionally the ensuing matters, demanding situations and customers, for you to determine these elements with the best power for modeling and exploring the functionality of ecosystems.
- Includes fresh updates from the sector of environmental genomics
- Provides information of advances of equipment and views in their use
- Contains a multidisciplinary evaluation of the environmental sciences together with taxonomy, ecology, evolution, and diversity
- Focuses at the influence of modern expertise advances in high-throughput sequencing
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Extra info for Insight on Environmental Genomics. High-throughput Sequencing
Genomic DNA sequencing from a single cell: “single cell genomics” Genomic DNA sequencing from a single cell, “single cell genomics” (SCG), is an innovative use of NGS. SCG is achieved through several steps: 1) cell isolation by dilution, micromanipulation or automated sorting by flow cytometry; 2) lysis of cells; 3) WGA (Whole Genome Amplification)-based amplification of DNA, mostly using the DNA polymerases Phi29 or Bst with the “multiple displacement amplification” (MDA) method; 4) NGS sequencing.
Sampling (1) and sequencing a monolocus marker (2) leads to a first species delimitation hypothesis (3 and 4). The application of an integrative approach to the same specimens, including an NGS multilocus analysis, can give rise to a new species delimitation hypothesis (5) eventually leading to a new description of species (6), © Nicolas Puillandre. zip 54 Insight on Environmental Genomics NGS approaches appear to be potential solutions to these issues. They enable the sequencing of many specimens as well as many genetic markers.
Genomic NGS and “single cell” methods, applied to environmental communities, can now be used to better describe the gene collections in microbial communities to infer taxons, horizontal transfers, interactions and adaptations in the microbial world. 5. 5. Towards integrative taxonomy Integrative taxonomy nowadays creates new opportunities for dialogue between pre-NGS taxonomy approaches and approaches that integrate NGS, such as the multilocus barcode [CRI 14]. Important research to test new hypotheses on the delineation of species is becoming possible.
Insight on Environmental Genomics. High-throughput Sequencing by Denis Faure, Dominique Joly