Download e-book for iPad: Multiple Detection in Size-Exclusion Chromatography by André M. Striegel

By André M. Striegel

ISBN-10: 0841220115

ISBN-13: 9780841220119

ISBN-10: 0841238782

ISBN-13: 9780841238787

A number of Detection in Size-Exclusion Chromatography offers a complete view of detection ideas which are used synergistically in size-exclusion chromatography (SEC). those concepts contain differential refractometry; static and dynamic gentle scattering; differential viscometry; fluorescence, ultraviolet, infrared, and nuclear magnetic resonance spectroscopy; electrospray ionization, matrix-assisted laser desorption ionization, chemical response interface, inductively coupled plasma, and tandem mass spectrometry; and dynamic floor pressure. This publication indicates how those suggestions supply precious molar mass, architectural, compositional, and thermodynamic details even if SEC is used because the sole separation procedure or as a part of a two-dimensional chromatographic set-up. within the latter case, the facility to split analytes in line with measurement, supplied by way of SEC, is augmented via separation in line with chemical composition, block size, etc., supplied by means of excessive functionality liquid chromatography, liquid chromatography on the serious situation, part fluctuation chromatography, or excessive osmotic strain chromatography.

A huge variety of functions are proven during the booklet, exemplifying either ordinary and artificial polymers. those comprise, yet aren't limited to, the research of lengthy- and short-chain branching in polyolefins; the research of methyl methacrylate polymers, copolymers, and oligomers; elemental speciation in environmental and organic samples; the characterization of oligo- and polysaccharides; the research of advanced protein combinations and of glycoproteins and protein-polymer conjugates; the research of polymer response kinetics; the decision of chemical and compositional heterogeneity; the characterization of semi-flexible artificial polymers and polypeptides; and the research of chromatographic band broadening.

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Although LS obviates the need for any column calibration, this type of fit is a 'hybrid' approach to data analysis. It extrapolates the absolute determination of M over the elution range where there is sufficient signal/noise in the LS data into the early and late ranges where the RI signal may still be good, but the LS data poor, as seen by the scattered M points at the beginning and end of the elution profile. This type of extrapolation is especially helpful at low values of M. This technique is self-consistent in that it uses a 'customcalibration' curve for each experiment which applies identically to the polymer being characterized.

Ann. , 1910,33, 1275 Debye, P. J. Phys. and Coll. H. J. Chem. ; Benoit, H. Can. J. N. J. Polym. D. , John Wiley & Sons, NY, 1975, ch. , Eds. C. Adv. Polym. , 1994, 114, 233 Kerker, M. F. J. App. Polym. H. ; Mark, H. J. Chem. R. J. Phys. D: Appl. F. F. Macromolecules, 1999, 32, 7055 Burchard, W. Adv. Polym. H. ; McClain, S. Macromolecules, 2000, 33. , R. E. Krieger, Malabar, Fla. Yamakawa, H. , Sects. , J. Chem. F. ch. 2, pp. 7-34, in Strategies in Size Exclusion Chromatography 1996, ACS ser. 635 , Eds.

Importantly, [η] is a direct measure of the ratio of a polymer's hydrodynamic volume V to its mass M. 56χ10 . The value of Φ changes with chain architecture and the perturbing effects of hydrodynamic interactions. As for , there is often a scaling law between [η] and M , of the form ν ν 21,27 2 [η] = G M Y (29) where γ is sometimes termed the Mark-Houwink exponent. ; ACS Symposium Series; American Chemical Society: Washington, DC, 2004. (30) 30 Knowledge of G and γ also allows computation of the viscosity averaged mass Μ which lies between M and M for γ<1.

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