Download e-book for kindle: Neuroendocrine Peptide Methodology by P. Michael Conn

By P. Michael Conn

ISBN-10: 0121851508

ISBN-13: 9780121851507

ISBN-10: 0323141897

ISBN-13: 9780323141895

Neuroendocrine Peptide method

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M. was supported by NIH predoctoral training grant GM07759. [3] NEUROPEPTIDE GENE TRANSCRIPTION 37 [3] Neuropeptide Gene Transcription in Central and Peripheral Nervous System Tissue by Nuclear Run-On Assay By J e f f r e y D. W h it e and E d m u n d F. L a G a m m a Introduction Rapid progress in molecular biological techniques has enabled the study of gene expression in a wide variety of neuroendocrine tissues. Through the use of dot blot,1 Northern gel,2 and in situ3 hybridization techniques, the mechanisms of accumulation or depletion of mRNA cod­ ing for specific neuroendocrine precursor proteins can now be elucidated.

M. was supported by NIH predoctoral training grant GM07759. [3] NEUROPEPTIDE GENE TRANSCRIPTION 37 [3] Neuropeptide Gene Transcription in Central and Peripheral Nervous System Tissue by Nuclear Run-On Assay By J e f f r e y D. W h it e and E d m u n d F. L a G a m m a Introduction Rapid progress in molecular biological techniques has enabled the study of gene expression in a wide variety of neuroendocrine tissues. Through the use of dot blot,1 Northern gel,2 and in situ3 hybridization techniques, the mechanisms of accumulation or depletion of mRNA cod­ ing for specific neuroendocrine precursor proteins can now be elucidated.

50 Thus, this viral system offers unique advantages of both stably trans­ fected cell lines and transient expression systems. The bovine papilloma viral-transformed cell lines are stable and therefore available for repeated experiments and the bovine papilloma viral vector containing the exoge­ nous gene is maintained in a homogeneous environment since it does not integrate into the chromosomal DNA. Different hybrid plasmids contain­ ing bovine papilloma virus sequences and pBR322 sequences are being constructed which can transform cells at high efficiency and serve as shuttle vectors between eukaryotic cells and bacterial cells.

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Neuroendocrine Peptide Methodology by P. Michael Conn


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